10 wells were used to create a gradient with increasing concentration of the drug. Material Your images originate from a ’96-well plate’, but you will work with a subset of 26 images with the following setup: 8 wells untreated (negative control) and 8 wells were treated with the maximum dose of 150nM (positive control). In this case how a protein is translocated from one subcellular compartment to another. The optimal goal of developing this type of image based screens is to use it to search for other, previously unknown drugs that have the same effect on the cell level, and may be of use for treatment of patients (possibly with less side effects than the known drug).Ĭhallenge Analyze how cells respond to treatment with a drug. We also want to optimize the image analysis to separate positive (treated with 150nM drug) and negative controls (untreated) as well as possible. We know that 150nM of a positive control drug causes the cells to transport the protein from the cytoplasm to the nucleus, but we do not know the lowest possible dose to see this effect. Scenario In this experiment we have human osteosarcoma cells where a ‘forkhead’-protein has been labeled with GFP (a green fluorescing protein). Download sample images and a description of experimental parameters from However, for this exercise the programs have already been installed on your computer, and can be found on ‘Gemensamt on 'IT-Pclab' (G:) → Program’.
#Cellprofiler analyst download software#
Starting software and download images CellProfiler and CellProfilerAnalyst can be downloaded from This webpage also provides tutorials, examples and forum where you can find answers questions you may have and get help. Computer exercise in CellProfiler & CellProfiler Analyst, 111129 Carolina Wählby, & Martin Simonsson,